Plasmid

Part:BBa_K3839042:Design

Designed by: Georgios Psarias   Group: iGEM21_Patras   (2021-10-21)


pcDNA3.4-TOPO + CYP2D6_204


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 723
    Illegal SpeI site found at 64
    Illegal PstI site found at 1309
    Illegal PstI site found at 2479
    Illegal PstI site found at 5098
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 64
    Illegal PstI site found at 1309
    Illegal PstI site found at 2479
    Illegal PstI site found at 5098
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 4048
    Illegal BglII site found at 8230
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 723
    Illegal SpeI site found at 64
    Illegal PstI site found at 1309
    Illegal PstI site found at 2479
    Illegal PstI site found at 5098
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 723
    Illegal SpeI site found at 64
    Illegal PstI site found at 1309
    Illegal PstI site found at 2479
    Illegal PstI site found at 5098
    Illegal NgoMIV site found at 3691
    Illegal NgoMIV site found at 4208
    Illegal NgoMIV site found at 5549
    Illegal NgoMIV site found at 5834
    Illegal AgeI site found at 3149
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1624
    Illegal BsaI.rc site found at 1904
    Illegal BsaI.rc site found at 3931
    Illegal BsaI.rc site found at 7362
    Illegal SapI site found at 2454
    Illegal SapI site found at 6279
    Illegal SapI.rc site found at 5398
    Illegal SapI.rc site found at 5608


Design Notes

HTML img Tag



References